91蝌蚪91九色-伊人久久影院-精品伊人-欧美福利在线观看-国户精品久久久久久久久久久不卡-色婷婷av一区二区三区之e本道-久久久久一-久久99热这里只频精品6学生-av免费试看-亚洲欧美一级-97超碰自拍-在线观看免费人成视频-亚洲欧美自拍另类-潘金莲性xxxxhd-缅甸黄色片-超碰久操-一级片高清-www.黄免费-中国精品毛片-草莓视频成人在线-国产第一页福利-秋霞午夜久久-精品久久免费观看-欧美性白人极品1819hd-丰满少妇熟乱xxxxx视频-国产一区你懂的-午夜网站视频-人成午夜视频-国产亚洲黄色片-国产男男gay做受ⅹxx高潮

【文獻標題】Ryanodine receptor 1 mediated dexamethasone-induced chondrodysplasia in fetal rats

【作者】Yi-zhong Wang，Qing-xian Li，Ding-Mei Zhang，et.al

【作者單位】武漢大學中南醫院（Zhongnan Hospital of Wuhan University）

【文獻中引用產品】

試劑-藏紅O

試劑-固綠FCF

【關鍵詞】Chondrodysplasia，Prenatal dexamethasone exposure，Ryanodine receptor 1，Transforming growth factor β，Endoplasmic reticulum stress

【DOI】doi.org/10.1016/j.bbamcr.2020.118791

【影響因子(IF)】4.66

【出版期刊】《BBA - Molecular Cell Research》

【產品原文引用】

Chemicals and reagents

Dex was obtained from Shuanghe Pharmaceutical Company (Wuhan, China). RYR1 agonist 4-chloro-3-ehylphenol (4-CEP) was purchased from Macklin (Shanghai, China), and RYR1 inhibitor dantrolene (Dan), ERS agonist tunicamycin (Ti), ERS inhibitor 4-phenylbutyric acid (4-PBA) and Fura-2 AM calcium fluorescent probe were purchased from Meilunbio Biotech (Dalian, China). Fluo-3 AM calcium fluorescent probe was purchased from Beyotime (Shanghai, China).DMEM F12 medium was purchased from Gibco Co., Ltd. (Grand Island,NY, USA). Safranin O and fast green was obtained from Hengyuan Biotech (Shanghai, China). TRIZOL was purchased from Invitrogen (Carlsbad, CA, USA). A reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) kit was purchased from Takara Biotechnology Co., Ltd. (Dalian, China). SYBR Green dye was obtained from Applied Biosystems, by Thermo Fisher Scientific (ABI, Foster City,CA, USA). All primers were synthesized by Sangon Biotech (Shanghai,China). Primary antibodies for RYR1 and 78 kDa glucose-regulated protein (GRP78) came from Sigma (St. Louis, MO, USA). The antibody for phosphorylated eukaryotic initiation factor 2A (p-EIF2A) was acquired from CST (Boston, MA, USA), the antibody for ATF-6 was acquired from Santa Cruz (Santa Cruz, CA, USA), the antibody for COL2A1 was acquired from Abbkine (Santa Cruz, CA, USA) and the antibody for ACAN was acquired from Bioss (Beijing, China). TGFβR1 antibody was purchased from ELK Biotechnology (Wuhan, China), and the antibodies for SMAD family member 2 (SMAD2), sex-determining region Y-box 9 protein (SOX9), ADAM metallopeptidase with thrombospondin type 1 motif 5 (ADAMTS5), and matrix metallopeptidase 13 (MMP13) were acquired from abcam (Cambridge, UK). The other reagents for the experiments were of analytical grade.

完整版PDF文獻請咨詢在線客服或者聯系我司業務員！

更多公司福利請關注“恒遠生物”VX公眾號！