91蝌蚪91九色-伊人久久影院-精品伊人-欧美福利在线观看-国户精品久久久久久久久久久不卡-色婷婷av一区二区三区之e本道-久久久久一-久久99热这里只频精品6学生-av免费试看-亚洲欧美一级-97超碰自拍-在线观看免费人成视频-亚洲欧美自拍另类-潘金莲性xxxxhd-缅甸黄色片-超碰久操-一级片高清-www.黄免费-中国精品毛片-草莓视频成人在线-国产第一页福利-秋霞午夜久久-精品久久免费观看-欧美性白人极品1819hd-丰满少妇熟乱xxxxx视频-国产一区你懂的-午夜网站视频-人成午夜视频-国产亚洲黄色片-国产男男gay做受ⅹxx高潮

【文獻標題】Effects of Taishan Pinus massoniana pollen polysaccharide on the subunit vaccine of Proteus mirabilis in birds

【作者】Guolin Cui，Shixun Zhong，Shifa Yang，et.al

【作者單位】山東農業大學（Shandong Agricultural University）

【文獻中引用產品】

雞分泌型免疫球蛋白A（sIgA）ELISA試劑盒

雞白細胞介素2（IL-2）ELISA試劑盒

【關鍵詞】Taishan Pinus massoniana pollen，polysaccharide，Subunit vaccine，Proteus mirabilis，Adjuvant，Effect

【DOI】dx.doi.org/10.1016/j.ijbiomac.2013.02.006

【影響因子(IF)】4.97

【出版期刊】《International Journal of Biological Macromolecules》

【產品原文引用】

2.4. Immunization

Threehundred1-day-oldchicks were randomlydividedintofive groups (n = 60 per group). Groups I–V were injected subcutaneously with 0.2 ml of WO-OMP vaccine, PP-OMP vaccine, TPPPS-OMP vaccine, OMP-only vaccine and physiological saline, respectively, at 3,7 and 12 days old. The final concentrations of OMP were 500 g/ml at the first vaccination, 1 mg/ml at the second vaccination and 2 mg/ml at the third vaccination. Seven days after the third vaccination, 30 chicks from each group were challenged with 100 LD50 P. mirabilis strain Q1 by oral administration.

On days 3, 7, 14, 21, 28, 35, 42 and 49 after the first vaccination,the peripheral blood and duodenum were sampled for analysis.The antibody titers were detected using indirect enzyme-linked immunosorbent assay (ELISA), T-lymphocyte proliferation rates were detected with flow cytometry and secreting-type IgA (SIgA)and interleukin-2 (IL-2) were measured using ELISA kits (Shanghai Hengyuan Bio-technology Co. Ltd.). The pathogenesis and survival status of chicks were clinically examined daily for 14 successive days after challenge. The mortality, morbidity and protective rates in each group were calculated as follows:

完整版PDF文獻請咨詢在線客服或者聯XI我司業務員！

更多公司福利請關注“恒遠生物”VX公眾號！