91蝌蚪91九色-伊人久久影院-精品伊人-欧美福利在线观看-国户精品久久久久久久久久久不卡-色婷婷av一区二区三区之e本道-久久久久一-久久99热这里只频精品6学生-av免费试看-亚洲欧美一级-97超碰自拍-在线观看免费人成视频-亚洲欧美自拍另类-潘金莲性xxxxhd-缅甸黄色片-超碰久操-一级片高清-www.黄免费-中国精品毛片-草莓视频成人在线-国产第一页福利-秋霞午夜久久-精品久久免费观看-欧美性白人极品1819hd-丰满少妇熟乱xxxxx视频-国产一区你懂的-午夜网站视频-人成午夜视频-国产亚洲黄色片-国产男男gay做受ⅹxx高潮

【文獻標題】Effects of RNA interference–mediated E‐selectin gene silencing on cell adhesionmolecule expression and cell‐cell adhesion in vascular endothelial cells in mice with immunologic contact urticaria

【作者】Zu‐Dong Meng，Xiao‐Lan Wang，Tian‐Ping Du，et.al

【作者單位】湖北醫(yī)科大學（Hubei University of Medicine）

【文獻中引用產(chǎn)品】

山羊抗兔抗體

【關鍵詞】cell adhesion molecule，cell‐cell adhesion，E‐selectin，gene silencing，immunologic contact urticaria，RNA interference，vascular endothelial cells

【DOI】doi.org/10.1002/jcb.27150

【影響因子(IF)】2.9

【出版期刊】《JOURNAL OF CELLULAR BIOCHEMISTRY》

【產(chǎn)品原文引用】

2.6 | Immunohistochemistry

Fourteen days after the model establishment, the endothelia cells were collected according to the aforementioned procedure. The VECs were fixed in 4% formalin for 24 hours, dehydrated by ethanol of grades 80%, 90%, and 100% and N‐butanol, and embedded in paraffin in a paraffin box at 60°C. After cutting the cells into 5‐μm serial sections, cell samples were allowed to stand at 45°C,collected, and dried at 60°C for 1 hour, followed by dewaxing by xylene. After dehydration, the sections were immersed in 3% H2O2 for 10 minutes and rinsed under distilled water. After antigen repair at high pressure for 90 seconds, the sections were cooled down to room temperature. Next, the sections were washed with PBS 3 times for 3 minutes each. Samples were blocked in 5% bovine serum albumin, incubated at 37°C for 30 minutes, and then added with primary antibody rabbit anti‐SELE (100 μL, 1:1000 to 1:4000, ab93952; Abcam,Cambridge, MA) for incubation at 4°C overnight. After washing the vascular endothelium samples with PBS, the samples were incubated with biotinylated goat anti‐rabbit secondary antibody (1:100, HY90046; Shanghai Hengyuan Biological Technology Co, Ltd, Shanghai, China) at 37°C for 30 minutes.

完整版PDF文獻請咨詢在線客服或者聯(lián)XI我司業(yè)務員！

更多公司福利請關注“恒遠生物”VX公眾號！